Cosmos 936 / Bion 4

C936-2.1

Title of Study

Quantitative Analysis of Selected Bone Parameters

Science Discipline

Musculoskeletal

Investigator
Institute
E.R. Morey-Holton
NASA-Ames Research Center
 
 
Co-Investigators
Institute
Turner, R.T.
American Lake VA Medical Center
Baylink, D.J.
American Lake VA Medical Center

Research Subjects

Rattus norvegicus (Wistar Rat)

5 Flight, 5 Centrifuged Males

Ground Based Controls

6 Vivarium, 5 Synchronous, 5 Centrifuged

Key Flight Hardware

Cosmos 936 Russian Hardware Suite

Objectives/Hypothesis

Changes in calcium homeostasis present a potential problem during prolonged space flight. Since mechanical forces imposed by muscle utilization and gravity influence bone turnover, prolonged recumbency and/or prolonged weightlessness with continuous hypercalciuria and bone loss could ultimately result in osteo- porosis. To better understand the effect of space flight and gravity on bone, the following parameters: formation and mineralization, resorption, length, density and pore size distribution, and mechanical properties, were studied in stationary and centrifuged space-flown rats both immediately following recovery and 25 days postflight.

Approach or Method

Density and pore size distribution were measured in the left femur by mercury porosimetry; mechanical parameters were evaluated with a standard torsion test machine. The rate of bone formation and resorption was determined in the left tibia by quantitative histological techniques; in addition, osteoblast and osteoclast cell number was determined.

Results

The data obtained demonstrate that: no gross change in endosteal bone resorption occurs during flight or postflight; mean periosteal bone formation rate decreases about 45% and is not corrected by centrifugation; the decrease in formation rate may be due, in part, to a cessation of bone formation which occurs sometime after the eleventh day of flight and continues until the second postflight day; although centrifugation did not correct the defect in periosteal bone formation rate during flight, it appears to hasten the recovery following flight; femur stiffness decreases about 30%; and centrifugation did correct the defect in bone mechanical proper- ties. All perturbations normalized by 25 days postflight. The reduction of mech- anical stress is probably not sufficient to account for the decreased rate of bone formation since a comparable decrease occurred in the flight centrifuged rats. However, the mechanical strength of the femur was not reduced in these ani- mals, and bone formation was apparently reinitiated immediately upon recovery in centrifuged rats, whereas it was delayed two to three days in flight rats.

Publications

Experiment Reference Number: C936-2.1

Holton, E.M.: Effects of Weightlessness on Bone and Muscle of Rats. Space Gerontology, NASA CP-2248, 1978, pp. 59-66.

Morey-Holton, E.R. et al.: Quantitative Analysis of Selected Bone Parameters. Final Reports of U.S. Experiments Flown on the Soviet Satellite Cosmos 936. S.N. Rosenzweig and K.A. Souza, eds., NASA TM-78526, 1978, pp. 135-178.

Spengler, D.M. et al.: Effects of Spaceflight on Bone Strength. Physiologist, supl., vol. 22, 1979, pp. S75-S76.

Spengler, D.M. et al.: Effects of Spaceflight on Structural and Material Strength of Growing Bone. Proceedings of the Society for Experimental Biology and Medicine, vol. 174, 1983, pp. 224-228.

Turner, R.T. et al.: Altered Bone Turnover During Spaceflight. Physiologist, supl., vol. 22, 1979, pp. S73-S74.

Turner, R.T. et al.: Spaceflight Results in Formation of Defective Bone. Proceedings of the Society for Experimental Biology and Medicine, vol. 180, 1985, pp. 544-549.

¥ = publication of related ground-based study