4. PROGRAMS, MISSIONS, AND PAYLOADS

Gemini 12

MISSION PROFILE: Gemini 12

Mission Duration: 4 days

Date: November 11-15, 1966

Life Sciences Research Objectives
To study development in fertilized frog eggs

Life Sciences Investigations
Cell/Developmental Biology (G12-1)

Organisms Studied
Rana pipiens (frog) eggs

Flight Hardware
Rana (frog) Egg Package

The four-day Gemini 12 mission was launched on November 11, 1966. Only one biological experiment was performed onboard the spacecraft. It was a reflight of an experiment that had flown on the Gemini 8 mission. The results of the Gemini 8 experiment were inconclusive because the mission had to be terminated prematurely.

Life Sciences Objectives

The objective of the experiment was to study the effect of microgravity on developmental processes.

Life Sciences Payload

     Organisms

     Eggs of the bullfrog Rana pipiens were used in the experiment.

     Hardware

     The hardware used was identical to that used in the experiment on Gemini 8, except that there was only one unit on this mission. The unit was mounted on the pilot's hatch.

Operations

Control studies and preflight procedures were similar to those performed for the Gemini 8 experiment.

     Fixative was released into individual egg chambers 40 minutes and 130 minutes after launch. Eggs in a third chamber were fixed shortly before re-entry. The eggs in the fourth chamber were left alive for comparison with the fixed specimens.

      The experiment equipment and specimens were removed from the spacecraft shortly after recovery.

Results

Development was normal, indicating that a gravitational field was not necessary for eggs to divide normally or to develop at later stages. However, as in the Gemini 8 mission, the eggs were fertilized on the ground and exposure to microgravity occurred only after the two-cell stage, which is thought to be critical for normal development.

Move to next section U.S. Biosatellite Program

Additional Reading

Anderson, M., J.A. Rummel, and S. Deutsch. BIOSPEX, Biological Space Experiments: A Compendium of Life Sciences Experiments Carried on U.S. Spacecraft. NASA TM-58217, June 1979.

Grimwood, J.M., B.C. Hacker, and P.J. Vorzimer. Project Gemini Technology and Operations: A Chronology. NASA Historical Series, NASA SP-4002, 1969.

Hacker, B.C. On the Shoulders of Titans: A History of Project Gemini. NASA SP-4203, 1977.

NASA. Spaceflight: The First 30 Years. NASA NP-150, December 1991.

Young, R.S. Gravity and Embryonic Development. Life Sciences and Space Research XIV, Akademie-Verlag, Berlin, 1976, pp. 69-75.

Young, R.S. and J.W. Tremor. Weightlessness and the Developing Frog Egg. Life Sciences and Space Research VI. North Holland Publishing Company, Amsterdam, 1968, pp. 87-93.

Zeitler, E.O. and T.G. Rogers. The Gemini Program Biomedical Sciences Experiments Summary. NASA TM-X-58074, September 1971.